The influence of MgCl2 and APB on the light-induced potassium changes and the ERG b-wave of the isolated superfused rat retina
نویسندگان
چکیده
The ERG and the extracellular potassium concentration, [K+]o, of the isolated superfused rat retina were measured in a physiological solution and in solutions containing 10 mM MgCl2 or 100 mu M APB. MgCl2 nearly abolished the b-wave, but the light-induced distal [K+]o increase was enlarged from 0.13 +/- 0.05 to 0.28 +/- 0.08 mM. There was also an increase in the light-induced [K+]o in the proximal retina. APB abolished the b-wave completely, and the distal light-induced [K+]o increase was then replaced by a [K+]o decrease. Upon return to the control solution, there was a larger transitory [K+]o increase than under control conditions, and this occurred before the b-wave had returned. Under these experimental conditions, the distal [K+]o increase could not be correlated with the b-wave, and so the Muller cells are unlikely to be the main source of the rising phase of the b-wave. More probable sources of the b-wave are the on-bipolar cells with their metabotropic and ionotropic receptors, with only the latter apparently being blocked by MgCl2. The extracellular [K+]o changes, however, had an influence upon the slow potentials of the ERG.
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عنوان ژورنال:
- Vision Research
دوره 36 شماره
صفحات -
تاریخ انتشار 1996